Month: November 2016

Salt tolerant genetic loci in rice exposed

Rice is a staple food for over half of the world’s population. It is also the most salt-sensitive cereal crop, with losses in yield reaching up to 69%.

In a new study published in Nature Communications collaborators from King Abdullah University of Science and Technology (KAUST) and The Plant Accelerator®, Australian Plant Phenomics Facility investigated the early responses of rice plants to moderately-saline conditions and pinpointed new salt-tolerant genetic loci.

Project lead, Professor Mark Tester (KAUST), supervised PhD student Nadia Al-Tamini’s project which grew 297 indica and 256 aus rice varieties under low and high salinity. Using a technique called ‘high-throughput non-invasive phenotyping’ plants are moved on conveyor belts, imaged daily using digital cameras to monitor biomass and shoot development, and weighed to carefully measure transpiration levels (water use).

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Dr Bettina Berger (left) and Nadia Al-Tamimi (right) in The Plant Accelerator®

“The Plant Accelerator® allowed us to analyse numerous aspects of the growth of multiple plants simultaneously,” says Professor Tester.

Using the facility’s cutting-edge technology, the researchers were able to show some genes, for example those connected with signaling processes, were important to plant growth in the first two to six days after salt application, while other genes became prominent later.

“This is perhaps the most astonishing aspect of this work – we can now obtain genetic details daily, pinpointing exactly when each locus comes into play in response to salinity,” says Professor Tester.

The results of this study could prove useful for breeding programs seeking to address yield and stress resistance to meet the demand of our increasing global population and climate challenges.

Congratulations to everyone involved in this study!!

Find the full articlewww.nature.com/articles/ncomms13342

More on Nadia Al-Tamini’s story:  https://blog.plantphenomics.org.au/2015/02/24/saudi-arabian-students-joins-plant-accelerator-team-to-investigate-salinity-tolerance-in-rice/

Professor Mark Tester, Plant Science Associate Director of the Center for Desert Agriculture Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology, Saudi Arabia, mark.tester@kaust.edu.sa, www.kaust.edu.sa/en/study/faculty/mark-tester

Dr Bettina Berger, Scientific Director, The Plant Accelerator, Australian Plant Phenomics Facility, University of Adelaide, www.plantphenomics.org.au, bettina.berger@adelaide.edu.au

Life is better with a “fun-gi”

Fungi colonise the roots of all cereal crops in a mutually beneficial association where the plant benefits from greater stress tolerance through improved water and mineral intake in exchange for carbohydrates for the fungi. The challenge in managing crop productivity and stress resilience is the unpredictability of plant growth responses when exposed to the fungi. It is possible to have too much or too little of a good thing! How do we get it right to avoid a detrimental impact on the plant and future crop production?

PhD student Rohan Riley, from Western Sydney University, is attempting to explain this unpredictability in terms of resource limitation by introducing fungal communities to plants which are isolated from soils containing high or low levels of salinity and analysing the effects on plant stress at the phenotypic level. He is undertaking his research at The Plant Accelerator® after being awarded a Postgraduate Student Internship Grant with the Australian Plant Phenomics Facility (APPF) in 2015.

”Using daily phenotyping following the application of salt stress and controlled watering-to-weight in The Plant Accelerator® allowed for an unprecedented resolution and range of plant genetic changes in response to combinations of nutrient level, salinity and two different fungal communities that would not otherwise be achievable in a regular greenhouse,”says PhD student, Rohan Riley.

rohan_brachy

”As a PhD student with limited experience in greenhouse experiments, the high controlled growth conditions, large-scale automation, digital imaging and software technology (high-throughput phenotyping) at The Plant Accelerator® provided me with the work-space, expertise and technical support to make a complicated experiment possible,”says Rohan.

The grain model Brachypodium distanchion was chosen to provide the greatest ease of knowledge transfer into many other crop plants with the view to developing future crops with greater resistance to environmental changes.

“It has been an amazing experience to conduct this experiment at The Plant Accelerator®. I am walking away from the facility with a big smile on my face, an incredible dataset for my PhD research and invaluable experience in greenhouse based plant research,’ says Rohan.

Thank you Rohan for being such a “fun guy” and a great addition to the team. It’s been a pleasure hosting you at The Plant Accelerator®!

Applications for the next round of APPF Postgraduate Student Internship Awards close on 30 November 2016.

To find out more about Rohan’s research:  https://www.researchgate.net/profile/Rohan_Riley

To find out more about Postgraduate Student Internship Grants, Scholarships and other programs at the Australian Plant Phenomics Facility:  http://www.plantphenomics.org.au/education/

Inaugural AusPheno Conference

The inaugural AusPheno Conference 2016 organised by the APPF, was held in September at the CSIRO Discovery Centre. The conference brought together 155 Australian and international plant scientists from the public and private sector for a week of talks and activities.

blog-2016-ausphenoCombined with the 5th International Controlled Environment Conference, topics reviewed the latest phenomics technologies and their application and presented research findings in plant phenomics, including environmental controlled and field phenotyping, remote sensing, root phenotyping and genome to phenome analysis to name a few.

The APPF launched ‘Plant Phenomics Australia’ during the conference, a new network to provide plant phenomics researchers and other stakeholders from across Australia with a platform to interact, collaborate, share ideas and best practice in phenomics research.

The conference was a resounding success with delegates enjoying the quality, range and depth of talks presented during the week. More information including copies of the talks presented can be accessed from the conference website.

The next AusPheno conference will be held in 2018, more details will be posted in 2017.

Related blog here