plant nutrients

Growing rice faster – uncovering the triggers behind early canopy closure

By combining high-resolution image-based phenotyping with functional mapping and genome prediction, a new study has provided insights into the complex genetic architecture and molecular mechanisms underlying early shoot growth dynamics in rice.

The more rapidly leaves of a plant emerge and create canopy closure, the more successful the plant, in establishment, resource acquisition and ultimately yield. An early vigor trait is particularly important in aerobic rice environments, which are highly susceptible to water deficits. The timing of developmental ‘triggers’ or switches that initiate tiller formation and rapid exponential growth are a critical component of this trait, however, searching for the switch that initiates this growth has proven challenging due to the complex genetic basis and large genotype-by-environment effect, and the difficulty in accurately measuring shoot growth for large populations.

“The availability of large, automated phenotyping platforms, such as those at Australian Plant Phenomics Facility (APPF), allow plants to be non-destructively phenotyped throughout the lifecycle in a controlled environment, and provide high resolution temporal data that can be used to examine these important developmental switches,” said PhD student, Malachy Campbell.

Malachy and team, including Bettina Berger and Chris Brien from the APPF, phenotyped a panel of ~360 diverse rice accessions throughout the vegetative stage (11-44 day old plants) at The Plant Accelerator® at APPF. A mathematical equation was used to describe temporal growth trajectories of each accession. Regions of the genome that may regulate early vigor were inferred using genome-wide association (GWA) mapping. However, many loci with small effects on shoot growth trajectories were identified, indicating that many genes contribute to this trait. GWA, together with RNA sequencing identified a gibberellic acid (GA) catabolic gene, OsGA2ox7, which could be influencing GA levels to regulate vigor in the early tillering stage.

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Dr Malachy Campell in The Plant Accelerator® at the Australian Plant Phenomics Facility’s Adelaide node

For some traits where genetic variation is controlled by a small number of loci, breeders can use MAS to identify individuals carrying the favourable locus/loci for the given trait, and select them for the next generation. For complex traits that are regulated by many loci, it becomes very difficult to detect loci that are associated with the trait. However, an alternative approach, genomic selection (GS), considers the total genetic contribution of all loci to the given trait. With this approach, loci across the genome can be used to predict the performance of individuals that have not yet been phenotyped (i.e. those in future generations). Since many loci were found to be contributing to early vigor, the team explored the possibility of using GS for improving this trait. Shoot growth trajectories could be predicted with reasonable accuracy, with greater accuracies being achieved when a higher number of markers were used. These results suggest that GS may be an effective strategy for improving shoot growth dynamics during the vegetative growth stage in rice. The approach of combining high-resolution image-based phenotyping, functional mapping and genome prediction could be widely applicable for complex traits across numerous crop species.

Read the full paper, published in The Plant Genome, here. (doi:10.3835/plantgenome2016.07.0064).

This is your chance! An invaluable opportunity to access phenotyping capabilities to further your plant science research

Do you have an exceptional plant science research project destined to deliver high impact outcomes for Australian agriculture? Do you need access to plant phenotyping capabilities?

The Phenomics Infrastructure for Excellence in Plant Science (PIEPS) scheme was announced in May and is open to all publicly funded researchers. Emphasis is placed on novel collaborations that bring together scientists preferably from different disciplines (e.g. plant physiology, computer science, engineering, biometry, quantitative genetics, molecular biology, chemistry, physics) and from different organisations, within Australia or internationally, to focus on problems in plant science.

The PIEPS scheme involves access to phenotyping capabilities at the Australian Plant Phenomics Facility (APPF) at a reduced cost to facilitate exceptional research projects. Researchers will work in partnership with the APPF to determine experimental design and optimal use of the equipment. Our team includes experts in agriculture, plant physiology, biotechnology, genetics, horticulture, image and data analysis, mechatronic engineering, computer science, software engineering, mathematics and statistics.

Applications are assessed in consultation with the APPF’s independent Scientific Advisory Board. Selection is based on merit.

Don’t miss this an outstanding opportunity to gain access to invaluable expertise and cutting edge technology to accelerate your research project and make a real impact in plant science discovery.

Applications close:  30 September 2017

For more information and to apply:  APPF Phenomics Infrastructure for Excellence in Plant Science (PIEPS)

To find out how we can support your research, contact us.

Travel grant opportunity to attend the 34th Annual Root Biology Symposium

IPPN Root Phenotyping Working Group
Travel Grant for Researchers Using Phenotyping
IPG 2017, 34th Annual Root Biology Symposium
Columbia, Missouri, USA
7-9 June 2017

The IPPN Root Phenotyping Working Group (RPWG) encourages mobility among researchers and enhances international contacts between research groups. With this sponsorship grant RPWG  supports participation of Early Career Researchers at the IPG 2017, 34th Annual Root Biology Symposium.

  • Up to four grants of 500 EUR per researcher can be awarded.
  • 1 May 2017

Conditions:

  • You are affiliated with a university or a research institution and you are an early career scientist, PhD student, or postdoc who finished his PhD no later than ten years ago.
  • Please fill in the travel grant application and submit it to Saoirse Tracy.
  • The applications will be evaluated by the RPWG Board.

Getting to the root of plant zinc health

Sunlight and water are two obvious requirements essential for healthy growth of plants, but did you know that zinc is also a vital ingredient? Zinc is a critical nutrient in hundreds of enzyme systems which are necessary for normal plant function. Zinc is also critical for human health – in fact, zinc is involved in more body functions than any other mineral.

Plants get zinc from the soil via their root systems. This uptake of nutrients is enhanced in many plants by mycorrhizal fungi which colonise the roots, creating a vast connection between the plant roots and the soil around them. Mycorrhizal fungi effectively increase the surface area of the roots, collecting nutrients from the soil beyond the reach of plant roots alone, and transfer these nutrients back to the plant.

Scientist, Dr Stephanie Watts-Williams, wants to find out how such mycorrhizal fungi can improve the zinc nutrition of plants, and subsequently impact on human health – particularly in countries where zinc malnutrition is a serious issue.

Read on here about Stephanie and her research at The Plant Accelerator®, Australian Plant Phenomics Facility, and other Waite Research Precinct partners.

Discover more about Stephanie’s research here or find her on Twitter:  @myco_research

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Dr Stephanie Watts-Williams at The Plant Accelerator®, Australian Plant Phenomics Facility